Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric techniquel products using flow injection spectrophotometric technique

Determination of tetracycline hydrochloride in some pharmaceutical products using flow injection spectrophotometric technique


Shirwan Omer baban 1 Rasul Jameel Ali 2



1 Chemistry Department, College of Education Science, Salahaddin University-Hawler, Iraqi-Kurdistan Region 2 Ministry of
Agriculture and Water Resources, Erbil
2 Clinical Biochemistry department, College of Health Sciences, Hawler Medical University, Hawler / Iraqi-Kurdistan Region
E- shirwan_baban@yahoo.co.uk




Article info

Original: 17 April. 2015

Revised:11 August. 2015

Accepted:30 August.  2015

Published online: 20 March.  2016





Key Words:

Tetracycline
hydrochloride, 2-
aminopyridine, flow
injection
spectrophotometric


Abstract
A simple, rapid, and sensitive flow injection spectrophotometric system for
determination of tetracycline hydrochloride (TCH) in pharmaceutical products. The
method is based on diazotization of 2-aminopyridine and subsequent coupling with
tetracycline hydrochloride in alkaline medium. The formed azo-dye showed maximum
absorbance at 388 nm and the calibration curve in the linear range of 25.0-150.0 μg/ml
of tetracycline hydrochloride with correlation coefficient 0.9988, while the detection
limit was 10 μg/ml and sample frequency 123 samples/hour. The accuracy and
precision data for determination of TCH by flow injection analysis method for triplicate
injection at three concentration levels(25, 80 and 150 μg/ml) the relative standard
deviation (RSD %) are 1.04, 0.247 and 1.75%, and relative error percent (Erel) are -
3.92, +1.06 and -1.9%. The proposed method was successfully applied to the
determination of tetracycline hydrochloride in pharmaceutical products. The results
obtained were in good agreement with those obtained by reference method.







Ċ
Ramyar Abdolrahman Taimur Ismail,
Mar 20, 2016, 4:49 AM